Molecular cloning of the major surface antigen of leishmania

Cloning and Expression of Leishmania major Superoxide Dismutase B1: A Potential Target Antigen for Serodiagnosis of Leishmaniasis

Cloning and sequencing of Toxoplasma gondii major surface antigen (SAG1) gene

  Genetic typing methods of T. gondii strains have been extensively perfected in recent years. From a technical point of view, many tools usable for genetic studied on single-copy loci have been used: RFLP, PCR-RFLP, sequencing, RAPD-PCR and isoenzyme analysis. We described the cloning and sequence analysis of the gene which encodes the major surface antigen (SAG1 or P30) of T. gondii. SAG1 is ...

Alginate Microsphere as a Delivery System and Adjuvant for Autoclaved Leishmania major and Quillaja Saponin: Preparation and Characterization

     The goal of this study was to prepare and characterize alginate microspheres as an antigen delivery system and adjuvant for immunization against leishmaniasis. Microspheres encapsulated with autoclaved Leishmania major (ALM) and Quillaja saponin (QS) were prepared by an emulsification technique and characterized for size, encapsulation efficiency and release profile of encapsulate...

Molecular Analysis of A2-genes Encoding Stage-specific S Antigen-like Proteins among Isolates from Iranian Cutaneous and Visceral Leishmaniasis

Objective(s) Leishmania can lead to a broad spectrum of diseases, collectively known as leishmaniasis. The A2 gene/ protein family could be one of the most eligible candidate factors of virulence in visceral leishmaniasis (VL). The previous results confirmed that in Leishmania infantum, several A2 proteins are abundantly expressed by the amastigote, but not the promastigote stage. As there are...

Molecular Cloning, Expression and Enzymatic Assay of Pteridine Reductase 1 from Iranian Lizard Leishmania

Background: Currently, there are no effective vaccines against leishmaniasis, and treatment using pentavalent antimonial drugs is occasionally effective and often toxic for patients. The PTR1 enzyme, which causes antifolate drug resistance in Leishmania parasites encoded by gene pteridine reductase 1 (ptr1). Since Leishmania lacks pteridine and folate metabolism, it cannot synthesize the pterid...

Preparation and In Vitro Characterization of Alginate Microspheres Encapsulated with Autoclaved Leishmania major (ALM) and CpG -ODN

Objective The goal of this study was to prepare and characterize alginate microspheres as an antigen delivery system and adjuvant for immunization against leishmaniasis. Materials and Methods Microspheres were prepared by an emulsification technique and characterized for size, encapsulation efficiency, and release profile of encapsulates. Selection of appropriate parameters (viscosity of al...